金亚

更新时间:2024-01-06 14:00

金亚,清华大学化学系博士,现任广东工业大学硕士生导师。

教育经历

1997.09-2002.07:清华大学化学系分析化学专业,获博士学位

1992.09-1997.07:清华大学化学系,物理化学及仪器分析专业,获学士学位

工作经历

2017.3至今:广东工业大学,生物医药研究院,教授

2012.10-2017.02:华南理工大学,生物科学与工程学院,副研究员/副教授

2005.04-2012.04:日本爱媛大学,理工学研究科,外国人研究员

2003.04-2005.03:日本爱媛大学理学部,日本学术振兴会(JSPS)外国人特别研究员

教学活动

已毕业硕士研究生6名,现指导博士研究生1名,硕士研究生1名。

主讲本科生必修课《生物化学及分子生物学》、《现代生化技术》;硕士研究生必修课《SCI文章写作与优秀外文科技文章导读》,硕士及博士研究生选修课《蛋白质组学分析技术及蛋白质组信息学》、《专业英语》等。

学术成果

承担项目

在研主持项目:广东工业大学引进人才项目1。

已结题主持项目:国家自然科学基金青年科学基金项目1、华南理工大学中央高校基本科研业务费重点项目1、广州市科技计划项目1、广东省发酵与酶工程重点实验室开放基金项目2、华南理工大学中央高校基本科研业务费面上项目1。

著作

Takashi Manabe,Ya Jin,in:Hughes AB(Ed.),Amino acids,Peptides and Proteins in Organic Chemistry:Volume 5:Analysis and Function of Amino Acids and Peptides,Wiley-VCH Verlag GmbH & Co. KGaA ,Weinheim 2011,Chapter 13 “Two-dimensional gel electrophoresis and protein/polypeptide assignment”,pp. 439-462.

论文

1.Meiling Wen,Ya Jin*,Hao Zhang,Xiaoou Sun,Yihe Kuai,Wen Tan*,Proteomic Analysis of Rat Cerebral Cortex in the Sub-Acute to Long-Term Phases of Focal Cerebral Ischemia-Reperfusion Injury. J. Proteome Res,2019,online(DOI:10.1021/acs.jproteome.9b00220).

2.Ya Jin*,Meiling Wen,Qi Yuan,Jun Zhang,Wen Tan*,Beneficial effects of Coomassie staining on proteomic analysis employing PAGE separation followed with whole-gel slicing,in-gel digestion and quantitative LC-MS/MS.J. Chromatogr. B2019,1110-1111,25-35.

3.Ya Jin*,Jun Zhang,Takashi Manabe,Wen Tan*,Comparison of the performance of 1D SDS-PAGE with nondenaturing 2DE on the analysis of proteins from human bronchial smooth muscle cells using quantitative LC-MS/MS.J. Chromatogr. B2019,1105,193-202.

4.Meiling Wen,Ya Jin*,Takashi Manabe,Shumin Chen,Wen Tan*,A comparative analysis of human plasma and serum proteins by combining native PAGE,whole-gel slicing and quantitative LC-MS/MS:Utilizing native MS-electropherograms in proteomic analysis for discovering structure and interaction-correlated differences,Electrophoresis,2017,38(24),3111–3123.

5.Shumin Chen,Meiling Wen,Shujie Bu,Ahui Wang,Ya Jin*,Wen Tan*,Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and quantitative LC-MS/MS,Electrophoresis,2016,37(23-24),3126-3136.

6.Lixian Cheng,Hui Shi,Yan Jin,Xiaoxi Li,Jinshun Pan,Yimei Lai,Yan Lin,Ya Jin,Gaurab Roy,Allan Zhao,Fanghong Li. Adiponectin Deficiency Leads to Female Subfertility and Ovarian Dysfunctions in Mice.Endocrinology2016,157(12),4875-4887.

7.Ahui Wang,Xiaoou Sun,Qing Liu,Tingting Hu,Ting Han,Ya Jin*,Wen Tan*,The protective effects of Sildenafil on transverse aortic constriction induced cardiac hypertrophy in rats.Journal of Guangdong College of Pharmacy(China),2016,32(4),475-480.

8.Jie Wu,Yiguang Tian,Shanping Wang,Marco Pistolozzi,Ya Jin,Ting Zhou,Gaurab Roy,Ling Xu,Wen Tan,Design,synthesis and biological evaluation of bambuterol analogues as novel inhibitors of butyrylcholinesterase.Eur J Med Chem,2016,126,61-71.

9.Ya Jin,Jin Chen,Ahui Wang,Jun Zhang,Shumin Chen,Takashi Manabe,Wen Tan*,Analysis of low-density lipoprotein-associated proteins using the method of digitized native protein mapping,Electrophoresis,2016,37(14),2063-2074.

10.Ya Jin,Qi Yuan,Jun Zhang,Takashi Manabe,Wen Tan,Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 2. Similarity search between protein maps for the analysis of protein complexes,Electrophoresis,2015,36(17),1991-2001.

11.Ya Jin,Jun Zhang,Qi Yuan,Takashi Manabe,Wen Tan,Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 1. Preparation of more than 4000 native protein maps,Electrophoresis,2015,36(15),1711-1723.

12.Jun Zhang,Ya Jin*,Yanrui Ye,Wen Tan,Technologies in G-protein-coupled receptor-ligand binding assays.Chinese Journal of Pharmaceutical Analysis,2015,35(1),1-7.

13.Ya Jin,Shujie Bu,Jun Zhang,Qi Yuan,Takashi Manabe,Wen Tan,Native protein mapping and visualization of protein interactions in the area of human plasma high-density lipoprotein by combining nondenaturing micro 2DE and quantitative LC-MS/MS,Electrophoresis,2014,35(14),2055-2064.

14.Takashi Manabe,Ya Jin,Performance of non-denaturing micro 2-DE followed by third-dimension SDS-PAGE in the analysis ofEscherichia colisoluble proteins,Electrophoresis,2011,32(2),300-309.

15.Takashi Manabe,Ya Jin,Analysis ofE. colisoluble proteins by non-denaturing micro 2-DE/3-DE and MALDI-MS-PMF,Electrophoresis,2010,31(16),2740-2748.

16.Takashi Manabe,Ya Jin,Assignment of human plasma proteins on non-denaturing micro 2-DE gels using MALDI-MS.Seibutsu Butsuri Kagaku,2010,54(1),31-34.Japanese.

17.Ya Jin,Takashi Manabe. Analysis of polyethylene glycol-fractionated high-molecular-mass proteins in human plasma by non-denaturing micro 2-DE and MALDI-MS PMF,Electrophoresis,2009;30(20),3613-3621.

18.Ya Jin,Takashi Manabe. Differences in protein distribution between human plasma preparations,EDTA-plasma and heparin-plasma,analyzed by non-denaturing micro 2-DE and MALDI-MS PMF,Electrophoresis,2009;30(6),931-938.

19.Ya Jin,Takashi Manabe. Performance of agarose IEF gels as the first dimension support for non-denaturing micro 2-DE in the separation of high-molecular-mass plasma proteins and protein complexes,Electrophoresis,2009;30(6),939-948.

20.Takashi Manabe,Ya Jin,Noncovalent interactions in human plasma proteins analyzed by the comparison of nondenaturing and denaturing micro-2-D gel electrophoresis patterns after polypeptide assignment using matrix-assisted laser desorption/ionization-mass spectrometry and peptide mass fingerprinting,Electrophoresis,2008;29(12),2672-2688.

21.Takashi Manabe,Ya Jin,Nao Yamaguchi,Tetsuo Sugiyama,Kohei Ikari. Cleavage of fibrinogen alpha chains during isoelectric focusing of human plasma under non-denaturing conditions analyzed by micro two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry.Journal of Electrophoresis,2007;51(3/4),27-34.

22.Takashi Manabe,Ya Jin,Analysis of protein/polypeptide interactions in human plasma using nondenaturing micro-2-DE followed by 3-D SDS-PAGE and MS,Electrophoresis,2007;28(12),2065-2079.

23.Takashi Manabe,Ya Jin,Osamu Tani.Assignment of human plasma polypeptides on a nondenaturing 2-D gel using MALDI-MS and PMF and comparisons with the results of intact protein mapping,Electrophoresis,2007;28(5),843-863.

24.Ya Jin,Takashi Manabe.Alkaline extraction of human plasma proteins from nondenaturing micro-2-D gels for protein/polypeptide mass measurement and peptide mass fingerprinting using MALDI-TOF MS,Electrophoresis,2007;28(3),449-459.

25.Maki Kamakura,Keiko Morisawa,Hideyuki Komi,Ayako Tomatani,Fumiji Saito,YukoKonishi,Ya Jin,Takashi Manabe,Masayuki Kuroda,Shosuke Imai,Hiroyuki Mizuguchi,Taketoshi Taniguchi. Regulation of IL-27p28 gene by lipopolysaccharide in dendritic DC2.4 cells.Biochemical and Biophysical Research Communications,2006;349(4),1372-1377.

26.Ya Jin,Takashi Manabe. Direct targeting of human plasma for matrix-assisted laser desorption/ionization and analysis of plasma proteins by time of flight-mass spectrometry,Electrophoresis,2005;26(14),2823-2834.

27.Ya Jin,Takashi Manabe.High-efficiency protein extraction from polyacrylamide gels for molecular mass measurement by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry,Electrophoresis,2005;26(6),1019-1028.

28.Takashi Manabe,Ya Jin,Alkaline cleavage of covalent bonds in chicken insulin and bovine-lactalbumin analyzed by matrix-assisted laser desorption/ionization-mass spectrometry,Electrophoresis,2005;26(1),257-267.

29.Ya Jin,Guo-an Luo,Numerical calculation of the electroosmotic flow at the cross region in microfluidic chips,Electrophoresis,2003;24(7-8),1242-1252.

30.Ya Jin,Guo-an Luo,Fabrication of the microfluidic chips with integrated ultra-micro electrodes and its application in on-chip electrochemical detection.Chemical Journal of Chinese Universities,2003,24(7),1180-1184.Chinese.

31.Ya Jin,Guoan Luo,Tomoko Oka,Takashi Manabe.Estimation of isoelectric points of human plasma proteins employing capillary isoelectric focusing and peptide isoelectric point markers,Electrophoresis,2002;23(19),3385-3391.

32.Ya Jin,Guo-an Luo,Fabrication and applications of an interface to couple microfluidic chips with electrospray ionization mass spectrometer.Analytical Instrumentation(China),2003,(2),4-10.

33.Guang-hua Zhang,Zhao-ying Zhou,Yang-hua Tang,Guo-an Luo,Ya Jin,The development of micro chip electrophoresis analyser.Chinese Journal of Scientific Instrument,2002,(4),335-338.

34.Guang-hua Zhang,Zhao-ying Zhou,Yang-hua Tang,Guo-an Luo,Ya Jin,Design of abiochemicalsubstanceanalysissystem.Measurement & Control Technology(China),2002,21(4),10-12.

35.Yang-hua Tang,Xiong-ying Ye,Zhao-ying,Zhou,Ya Jin,Yan-ying Feng.Glass chips for capillary electrophoresis,micromachining,sample injection and separation.Chinese Journal of Analytical Chemistry,2001,29(5),606-610.

36.Ya Jin,Tao Wen,Yi-ming Wang,Guo-an Luo,Development and application of the detection technologies of integrated capillary electrophoresis chips.Modern Scientific Instruments(China),2001,(4),15-17.

37.Yang-hua Tang,Zhao-ying Zhou,Xiong-ying Ye,Yan-ying Feng,Ya Jin,Microfabrication of capillary electrophoresis chips.Microfabrication Technology(China),2001,(1),53-55.

38.Ya Jin,Guo-an Luo,Yang-hua Tang,Zhao-ying Zhou. Microfabrication of integrated capillary electrophoresis chips and on-chip sample injection and separation.Journal of Analytical Science(China),2001,17(2),148-152.

39.Ya Jin,Guo-an Luo,Development of technology and applications of microarrays.Acta Pharmaceutica Sinica(China),2000,35(3),235-240.

40.Ya Jin,Guo-an Luo,Ru-ji Wang.Development of integrated capillary electrophoresis chips.Se Pu(China),2000,18(4),313-317.

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